IRAK4 Antikörper (AA 38-54, AA 120-136)

Produktnummer ABIN372431

Der Kaninchen Polyklonal anti-IRAK4 Antikörper wird verwendet zum Nachweis von IRAK4 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für IRAK4 Antikörper (AA 38-54, AA 120-136) (ABIN372431)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRAK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IRAK4 Antikörper

Bindungsspezifität: AA 38-54, AA 120-136

Spezifität: This antibody recognizes Interleukin 1 Receptor-Associated Kinase 4 (IRAK4).

Kreuzreaktivität (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Aufreinigung: Protein G Chromatography.

Immunogen: A mixture of synthetic peptides containing amino acids 38-54 and 120-136 of Mouse IRAK-4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Andere Bezeichnung: IRAK4

Hintergrund: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members. The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified. IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64

Gen-ID: 51135

NCBI Accession: NP_001107654

UniProt: Q9NWZ3

Pathways: NF-kappaB Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades