TLR9 Antikörper (AA 268-284) (FITC)
Kurzübersicht für TLR9 Antikörper (AA 268-284) (FITC) (ABIN364233)
Target
Alle TLR9 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 268-284
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Marke
- IHC-plus™
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Spezifität
- Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.
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Aufreinigung
- Protein G purified
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Immunogen
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Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
Type of Immunogen: Synthetic peptide -
Isotyp
- IgG1
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Applikationshinweise
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Approved: Flo, ICC, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry (Intracellular): 0.1-2 μg/10^6 cells, cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 10 mM Tris, 150 mM sodium chloride, 0.05 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- avoid freeze thaw cycles. Sensitive to light.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
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- TLR9 (Toll-Like Receptor 9 (TLR9))
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Andere Bezeichnung
- TLR9
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Hintergrund
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Name/Gene ID: TLR9
Family: Toll-like Receptor
Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9 -
Gen-ID
- 54106
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UniProt
- Q9NR96
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Pathways
- TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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