TLR9 Antikörper (AA 268-284) (FITC)

Produktnummer ABIN364233

Dieses Anti-TLR9-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TLR9 in WB, IHC, FACS, ICC, IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Hund, Affe und Pferd.

Kurzübersicht für TLR9 Antikörper (AA 268-284) (FITC) (ABIN364233)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reaktivität: Human, Maus, Ratte, Hund, Affe, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR9 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-TLR9 Antikörper (FITC)

Bindungsspezifität: AA 268-284

Marke: IHC-plus™

Spezifität: Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry (Intracellular): 0.1-2 μg/10^6 cells, cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM Tris, 150 mM sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Sensitive to light.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Details zu TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Andere Bezeichnung: TLR9

Hintergrund: Name/Gene ID: TLR9
Family: Toll-like Receptor

Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9

Gen-ID: 54106

UniProt: Q9NR96

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades