MAP2K3 Antikörper (pSer189)

Produktnummer ABIN362138

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MAP2K3 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für MAP2K3 Antikörper (pSer189) (ABIN362138)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP2K3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MAP2K3 Antikörper

Bindungsspezifität: pSer189

Spezifität: The antibody detects endogenous level of MKK3 only when phosphorylated at serine189.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer189 (V-D-S (p) -V-A) derived from Human MKK3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Andere Bezeichnung: MKK3

Hintergrund: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Molekulargewicht: 40 kDa

NCBI Accession: NP_002747

UniProt: P46734

Pathways: MAPK Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2