Collagen IV Antikörper

Produktnummer ABIN343923

Der Kaninchen Polyklonal anti-Collagen IV Antikörper (ABIN343923) detektiert spezifisch Collagen IV in WB, ELISA, IHC, IHC (p), IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Rind (Kuh).

Kurzübersicht für Collagen IV Antikörper (ABIN343923)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-Collagen IV Antikörper

Spezifität: Collagen Type IV from human and bovine placenta.

Aufreinigung: Immunoaffinity purified

Immunogen:

Type of Immunogen: Native protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen IV