CD14 Antikörper

Produktnummer ABIN343180

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD14 in ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für CD14 Antikörper (ABIN343180)

Target: CD14

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: ELISA

Klon: BiG53

Produktdetails anti-CD14 Antikörper

Spezifität: Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.

Aufreinigung: Protein A affinity column

Immunogen: Monocytes of mouse

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.16

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Gen-ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades