Der Maus Monoklonal anti-CD14 Antikörper (Klon biG57) (ABIN343179) detektiert spezifisch CD14 in ELISA.
Dieser Antikörper reagiert spezifisch mit Proben aus Maus.
CD14
Reaktivität: Human
WB, IHC
Wirt: Maus
Monoclonal
LPSR-2397
unconjugated
Applikationshinweise
ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with bidistilled water
Konzentration
1 mg/mL
Buffer
PBS, pH 7.15
Konservierungsmittel
Without preservative
Lagerung
-20 °C,-80 °C
Target
CD14
Andere Bezeichnung
CD14
Hintergrund
Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.