CD14 Antikörper

Produktnummer ABIN343179

Dieses Anti-CD14-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD14 in ELISA. Geeignet für Maus.

Kurzübersicht für CD14 Antikörper (ABIN343179)

Target: CD14

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: ELISA

Klon: BiG57

Produktdetails anti-CD14 Antikörper

Spezifität: Recombinant mouse CD14. The antibody don't blocks LPS binding to membrane bound CD14.

Aufreinigung: Protein G affinity column

Immunogen: recombinant mouse CD14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.15

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht: ~50kDa

Gen-ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades