CD40 Antikörper

Produktnummer ABIN337317

Produktdetails anti-CD40 Antikörper

Target: CD40

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: IHC-plus™

Spezifität: Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.

Aufreinigung: Protein A purified

Immunogen:

Type of Immunogen: Native protein

Klon: LOB7-6

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (20 μg/mL), IHC, IHC-Fr (1 - 10 μg/mL), IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris buffered saline, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40 (CD40 Produkte)

Synonyme: Bp50 antikoerper, CDW40 antikoerper, TNFRSF5 antikoerper, p50 antikoerper, AI326936 antikoerper, GP39 antikoerper, HIGM1 antikoerper, IGM antikoerper, IMD3 antikoerper, T-BAM antikoerper, TRAP antikoerper, Tnfrsf5 antikoerper, TNFSF5 antikoerper, CD40 molecule antikoerper, CD40 antigen antikoerper, CD40 antikoerper, Cd40 antikoerper

Hintergrund: Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5

Gen-ID: 958

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints