Collagen IV Antikörper

Produktnummer ABIN335405

Der Maus Monoklonal Anti-Collagen IV Antikörper wurde für WB, IHC, IHC (fro), IHC (p) und ICC validiert. Er ist geeignet, Collagen IV in Proben von Human, Ratte, Schwein und Rind (Kuh) zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für Collagen IV Antikörper (ABIN335405)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human, Ratte, Schwein, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: LP46

Produktdetails anti-Collagen IV Antikörper

Spezifität: Human, rat, pig, cow.

Aufreinigung: Purified

Immunogen: 1043 is a mouse monoclonal IgG2b antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1043 recognizes the extracellular - basement membranes. 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Collagen IV Antikörper (ABIN335405)

Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." in: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." in: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." in: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." in: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV