TLR4 Antikörper (AA 238-609)

Produktnummer ABIN334262

Der Maus Monoklonal Anti-TLR4 Antikörper wurde für FACS, IHC, ELISA und IHC (p) validiert. Er ist geeignet, TLR4 in Proben von Human zu detektieren.

Kurzübersicht für TLR4 Antikörper (AA 238-609) (ABIN334262)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 1E11

Produktdetails anti-TLR4 Antikörper

Bindungsspezifität: AA 238-609

Spezifität: Recognizes human Toll-like Receptor 4 (TLR4).

Aufreinigung: Purified

Immunogen: Recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000 - 1:4000), Flo, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Flow Cytometry: 3 μg/10e6 cellsOptimal dilutions to be determined by researcher.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4

Hintergrund: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gen-ID: 7099

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine