CD11b Antikörper (PE)

Produktnummer ABIN319554

Produktdetails anti-CD11b Antikörper (PE)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Maus, Kaninchen

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.

Klon: M1-70-15

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b (ITGAM Produkte)

Hintergrund: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gen-ID: 10090

UniProt: P05555

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation