CD11b Antikörper

Produktnummer ABIN319553

Der Ratte Monoklonal Anti-CD11b Antikörper wurde für FACS, IP, IHC (fro), Func und IHC (p) validiert. Er ist geeignet, CD11b in Proben von Human, Maus und Kaninchen zu detektieren.

Kurzübersicht für CD11b Antikörper (ABIN319553)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Maus, Kaninchen

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: M1-70-15

Produktdetails anti-CD11b Antikörper

Kreuzreaktivität (Details): Species reactivity (expected):Human, Rabbit.
Species reactivity (tested):Mouse.

Aufreinigung: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin-Niveau: Low Endotoxin

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Immunoprecipitation. Flow Cytometry: 1/50 - 1/100 (routinely tested in flow cytometry on mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level < 0.01EU/ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund: The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gen-ID: 10090

UniProt: P05555

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation