PPP1CA Antikörper

Produktnummer ABIN306640

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PPP1CA in WB, IP, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PPP1CA Antikörper (ABIN306640)

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PPP1CA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PPP1CA Antikörper

Spezifität: P16 INK4a

Kreuzreaktivität: Human

Kreuzreaktivität (Details): Human, other species not tested

Produktmerkmale: pp1a, rabbit anti protein phosphatase 1 alpha,The antibody is suitable to detect p16 in different tissues. Staining results may be cytoplasmatic or nuclear. Nuclear staining should be more specific. Human p16 INK4a (syn. CDKN2, MTS-1) protein is an inhibitor of Cyclin-Dependent-Kinases 4 and 6 (cdk4/cdk6). CDK4 is an important enzyme for the progression of the cell cycle during the G1-Phase. By it's inhibitory action p16 is an important regulator in the cell cycle. The gen encoding for p16 is deleted in many tumour cell lines. In adenocarcinoma of the cervix uteri usually p16 levels are increased. Endocervical p16-overexpression is mostly associated with "high risk" HPV-Typing. In other tumour types as squamous epithelial carcinoma in cervix uteri and head and neck carcinoma p16 expression usually is reduced.

Aufreinigung: Protein A affinity purification.

Immunogen: Recombinant human p16 INK4a molecule,Monoclonal antibody DCS-50 is produced after immunisation of Balb/c mice with the full length recombinant p16 INK4a protein of human origin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Pre-treatment with Unmasking Fluid C (Citrate Buffer) or Unmasking Fluid G at 96-100 degree of Celsius is recommended for paraffin sections.,IHC (C,P), IP,60 min at RT,1:10

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: Protein A affinity purified antibody lyophilized from PBS pH 7.4 with BSA and Na-Azide 0.09 % .

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C

Details zu PPP1CA

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Andere Bezeichnung: Protein Phosphatase 1 alpha

Pathways: M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism