NF-kB p65 Antikörper (N-Term)

Produktnummer ABIN3043699

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NF-kB p65 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 43+ Publikationen erwähnt.

Kurzübersicht für NF-kB p65 Antikörper (N-Term) (ABIN3043699)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: AA 116-131, N-Term

Verwendungszweck: Anti-NF-kB p65/RELA Antibody Picoband®

Sequenz: AISQRIQTNN NPFQVP

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NF-kB p65/RELA Antibody (ABIN3043699). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NF-kB p65, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Deloukas, P., van Loon, A. P. G. M. : Genomic organization of the gene encoding the p65 subunit of NF-kappa-B: multiple variants of the p65 protein may be generated by alternative splicing. Hum. Molec. Genet. 2: 1895-1900, 1993. 2. Nickols, J. C., Valentine, W., Kanwal, S., Carter, B. D. : Activation of the transcription factor NF-kappa-B in Schwann cells is required for peripheral myelin formation. Nature Neurosci. 6: 161-167, 2003. 3. Zhong, H., Voll, R. E., Ghosh, S. : Phosphorylation of NF-kappa B by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Molec. Cell 1: 661-671, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-NF-kB p65 Antikörper (ABIN3043699)

"Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, (2018) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "[Corrigendum] Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6926, (2018) (PubMed).

Wang, Liu, Wang, Wang, Tang, Jiang: "Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1282-1294, (2018) (PubMed).

Liu, Bao, Zeng, Wei: "Effects of resveratrol and genistein on nuclear factor‑κB, tumor necrosis factor‑α and matrix metalloproteinase‑9 in patients with chronic obstructive pulmonary disease." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4266-72, (2017) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Wu, Gao, Li, Yao, Wang, Chen, Li, Ma, Ji, Li: "Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2715718, (2017) (PubMed).

Xie, Zhong, Li: "Comment on "Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats"." in: Journal of diabetes research, Vol. 2016, pp. 7302620, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Yang, Gao, Wu, Yu, Li, Meng, Li, Yan, Jin: "Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 1975-1982, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).

Chen, Liu, Wang, Feng, Zhao, Shi, Liu: "Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury." in: Oxidative medicine and cellular longevity, Vol. 2015, pp. 106836, (2016) (PubMed).

Huang, Zhong, Zhang, Wang, Xu, Zhu, Wang, Hu, Zhao: "Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-κB pathway." in: Scientific reports, Vol. 5, pp. 12807, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Zuo, Zheng, Liu, Ma: "Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10653-61, (2016) (PubMed).

Wendan, Changzhu, Xuhong, Hongjing, Hong, Dongxia, Fang: "BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation." in: Stem cells international, Vol. 2016, pp. 3196071, (2016) (PubMed).

Wang, Li, Zhen, Xu, Sun: "Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy." in: International journal of clinical and experimental pathology, Vol. 8, Issue 12, pp. 15696-707, (2016) (PubMed).

Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 8219287, (2016) (PubMed).

Ge, Cao, Wang, Sun, Sun, Han, Cui, Liu: "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation." in: OncoTargets and therapy, Vol. 9, pp. 1011-21, (2016) (PubMed).

Li, Zhang, Qi, Zhang, Yi, Li, Zhang: "Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB." in: The journal of headache and pain, Vol. 17, pp. 49, (2016) (PubMed).

Wang, Su, Che, Zheng, Shi: "Penehyclidine ameliorates acute lung injury by inhibiting Toll-like receptor 2/4 expression and nuclear factor-κB activation." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1827-1832, (2016) (PubMed).

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA

Hintergrund:

Synonyms: Transcription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,NFKB3,

Background: The p65 (RELA) heterodimer is the most abundant form of NFKB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A (PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B p65 through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276.

Sequence Similarities: Contains 1 RHD (Rel-like) domain.

Molekulargewicht: 65 kDa

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine