Cyclin A Antikörper (AA 10-168)

Produktnummer ABIN3043481

Produktdetails anti-Cyclin A Antikörper

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Bindungsspezifität: AA 10-168

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cyclin-A2(CCNA2) detection. Tested with WB, IHC-P in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cyclin-A2(CCNA2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: cyclin A2
Protein Name: Cyclin-A2

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Cyclin A Antikörper (ABIN3043481)

Zhang, Chang, Zhao, Gao, Wang, Li, Zhang, Zhang, Xu: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).

Zhang, Chang, Li, Zhang, Xu: "The novel protein C3orf43 accelerates hepatocyte proliferation." in: Cellular & molecular biology letters, Vol. 22, pp. 21, (2017) (PubMed).

Wang, Ge, Chen, Hu, Li, Song, Xu, Ye: "A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21 (Waf1/CIP1) Expression." in: BioMed research international, Vol. 2015, pp. 304753, (2016) (PubMed).

Chen, Zhang, Liang, Zhou, Ding, Yang, Liu, Wu, Man, Zhang, Chen: "Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells." in: Cell communication and signaling : CCS, Vol. 12, pp. 18, (2014) (PubMed).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CCNA2 (CCNA2 Produkte)

Synonyme: CCN1 antikoerper, CCNA antikoerper, AA408589 antikoerper, Ccn-1 antikoerper, Ccn1 antikoerper, Ccna antikoerper, CycA2 antikoerper, Cyca antikoerper, cb671 antikoerper, chunp6924 antikoerper, wu:fi36f03 antikoerper, CYCA antikoerper, Cyclin-A2 antikoerper, ccna2 antikoerper, ccn1 antikoerper, ccna antikoerper, ccna1 antikoerper, Cyclin-A antikoerper, cyclinA antikoerper, cyclin A2 antikoerper, cyclin A2 S homeolog antikoerper, cyclin A2 L homeolog antikoerper, CCNA2 antikoerper, Ccna2 antikoerper, ccna2 antikoerper, ccna2.S antikoerper, ccna2.L antikoerper

Hintergrund: Cyclin A2, known as CCNA2, is mapped to 4q27. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. And Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. Phosphorylation of CDC6 and MCM4 by the cyclin A2-CDK2 complex prevents re-replication of DNA during the cell cycle.

Synonyms: CCN1 antibody|CCNA antibody|CCNA2 antibody|CCNA2_HUMAN antibody|Cyclin A2 antibody|Cyclin-A antibody|Cyclin-A2 antibody

Gen-ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA