Cyclin A Antikörper (AA 10-168)

Produktnummer ABIN3043481

Der Kaninchen Polyklonal Anti-Cyclin A-Antikörper wurde für WB, IHC, IF, FACS und ICC validiert. Er ist geeignet, Cyclin A in Proben von Human, Maus und Ratte zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für Cyclin A Antikörper (AA 10-168) (ABIN3043481)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Cyclin A Antikörper

Bindungsspezifität: AA 10-168

Verwendungszweck: Anti-Cyclin A2/CCNA2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cyclin A2/CCNA2 Antibody Picoband® (ABIN3043481). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Gong D, Ferrell JE (Sep 2010). "The roles of cyclin A2, B1, and B2 in early and late mitotic events". Mol. Biol. Cell 21(18): 3149-61. 2. Yam CH, Fung TK, Poon RY (Aug 2002). "Cyclin A in cell cycle control and cancer". Cell. Mol. Life Sci. 59 (8): 1317-26.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cyclin A Antikörper (ABIN3043481)

Zhang, Chang, Zhao, Gao, Wang, Li, Zhang, Zhang, Xu: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).

Zhang, Chang, Li, Zhang, Xu: "The novel protein C3orf43 accelerates hepatocyte proliferation." in: Cellular & molecular biology letters, Vol. 22, pp. 21, (2017) (PubMed).

Wang, Ge, Chen, Hu, Li, Song, Xu, Ye: "A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21 (Waf1/CIP1) Expression." in: BioMed research international, Vol. 2015, pp. 304753, (2016) (PubMed).

Chen, Zhang, Liang, Zhou, Ding, Yang, Liu, Wu, Man, Zhang, Chen: "Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells." in: Cell communication and signaling : CCS, Vol. 12, pp. 18, (2014) (PubMed).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CCNA2

Hintergrund:

Synonyms: Cyclin-A2,Cyclin-A,CCNA2,CCN1, CCNA,

Tissue Specificity: Ubiquitous.

Background: Cyclin A2, known as CCNA2, is mapped to 4q27. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. And Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated in the initiation and progression of DNA synthesis. Phosphorylation of CDC6 and MCM4 by the cyclin A2-CDK2 complex prevents re-replication of DNA during the cell cycle.

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Molekulargewicht: 55 kDa

Gen-ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA