SMAD4 Antikörper (C-Term)

Produktnummer ABIN3043341

Produktdetails anti-SMAD4 Antikörper

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Bindungsspezifität: AA 526-552, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 4(SMAD4) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: EIHLHRALQL LDEVLHTMPI ADPQPLD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 4(SMAD4) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: SMAD family member 4
Protein Name: Mothers against decapentaplegic homolog 4

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4 (526-552aa EIHLHRALQLLDEVLHTMPIADPQPLD), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat , The detection limit for Smad4 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-SMAD4 Antikörper (ABIN3043341)

Xu, Xu, Saud, Lu, Liu, Fang, Zhang, Hu, Li: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).

Cheng, Xiao, Ainiwaer, Wang, Wu, Mao, Yang, Bao: "Molecular responses of radiation-induced liver damage in rats." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2592-600, (2015) (PubMed).

Tang, Li, Yu, Gao, Liu, Chen, Xing, Liu, Yao: "Quercetin prevents ethanol-induced iron overload by regulating hepcidin through the BMP6/SMAD4 signaling pathway." in: The Journal of nutritional biochemistry, Vol. 25, Issue 6, pp. 675-82, (2014) (PubMed).

Chen, Kong, Wan, Xiao, Li, Wang, Lin, Wang: "Effects of huogu I formula (I) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head." in: Chinese journal of integrative medicine, Vol. 18, Issue 5, pp. 378-84, (2012) (PubMed).

Dong, Jia, Zhang, Li, Geng, Zhou, Li, Liu, Niu: "Emodin protects rat liver from CCl(4)-induced fibrogenesis via inhibition of hepatic stellate cells activation." in: World journal of gastroenterology, Vol. 15, Issue 38, pp. 4753-62, (2010) (PubMed).

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4 (SMAD4 Produkte)

Synonyme: smad4 antikoerper, DPC4 antikoerper, JIP antikoerper, MADH4 antikoerper, MYHRS antikoerper, AW743858 antikoerper, D18Wsu70e antikoerper, Madh4 antikoerper, XSmad4alpha antikoerper, Xsmad4 antikoerper, dpc4 antikoerper, jip antikoerper, madh4 antikoerper, xsmad4a antikoerper, SMAD family member 4 antikoerper, Smad4 protein antikoerper, SMAD family member 4, gene 1 L homeolog antikoerper, SMAD4 antikoerper, smad4 antikoerper, Smad4 antikoerper, smad4.1.L antikoerper

Hintergrund: SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFβ protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

Synonyms: (Small) Mothers Against Decapentaplegic antibody|Deleted in Pancreatic Carcinoma antibody|Deleted in pancreatic carcinoma locus 4 antibody|Deletion target in pancreatic carcinoma 4 antibody|DPC 4 antibody|DPC4 antibody|hSMAD4 antibody|JIP antibody|MAD homolog 4 antibody|MAD mothers against decapentaplegic Drosophila homolog 4 antibody|MAD mothers against decapentaplegic homolog 4 antibody|MADH 4 antibody|MADH4 antibody|Med antibody|Medea antibody|Mothers against decapentaplegic homolog 4 antibody|Mothers against DPP homolog 4 antibody|OTTHUMP00000163548 antibody|SMAD 4 antibody|SMAD family member 4 antibody|SMAD mothers against DPP homolog 4 antibody|Smad4 antibody|SMAD4_HUMAN antibody

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie