SMAD4 Antikörper (C-Term)

Produktnummer ABIN3043341

Dieses Anti-SMAD4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMAD4 in WB. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für SMAD4 Antikörper (C-Term) (ABIN3043341)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SMAD4 Antikörper

Bindungsspezifität: AA 526-552, C-Term

Verwendungszweck: Anti-Smad4 Antibody Picoband®

Sequenz: EIHLHRALQL LDEVLHTMPI ADPQPLD

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Smad4 Antibody Picoband® (ABIN3043341). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Hein, L., Altman, J. D., Kobilka, B. K. :Two functionally distinct alpha-2-adrenergic receptors regulate sympathetic neurotransmission. Nature 402: 181-184, 1999. 2. Hoehe, M. R., Berrettini, W. H., Lentes, K.-U.:Dra I identifies a two allele DNA polymorphism in the human alpha-2-adrenergic receptor gene (ADRAR), using a 5.5 kb probe (p ADRAR). Nucleic Acids Res. 16: 9070 only, 1988. 3. Yang-Feng, T. L., Kobilka, B. K., Caron, M. G., Lefkowitz, R. J., Francke, U.:Chromosomal assignment of genes for an alpha-adrenergic receptor (ADRAR) and for another member of this receptor family coupled to guanine nucleotide regulatory proteins (RG21). (Abstract) Cytogenet. Cell Genet. 46: 722-723, 1987.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-SMAD4 Antikörper (ABIN3043341)

Xu, Xu, Saud, Lu, Liu, Fang, Zhang, Hu, Li: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).

Cheng, Xiao, Ainiwaer, Wang, Wu, Mao, Yang, Bao: "Molecular responses of radiation-induced liver damage in rats." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2592-600, (2015) (PubMed).

Tang, Li, Yu, Gao, Liu, Chen, Xing, Liu, Yao: "Quercetin prevents ethanol-induced iron overload by regulating hepcidin through the BMP6/SMAD4 signaling pathway." in: The Journal of nutritional biochemistry, Vol. 25, Issue 6, pp. 675-82, (2014) (PubMed).

Chen, Kong, Wan, Xiao, Li, Wang, Lin, Wang: "Effects of huogu I formula (I) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head." in: Chinese journal of integrative medicine, Vol. 18, Issue 5, pp. 378-84, (2012) (PubMed).

Dong, Jia, Zhang, Li, Geng, Zhou, Li, Liu, Niu: "Emodin protects rat liver from CCl(4)-induced fibrogenesis via inhibition of hepatic stellate cells activation." in: World journal of gastroenterology, Vol. 15, Issue 38, pp. 4753-62, (2010) (PubMed).

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund:

Synonyms: Mothers against decapentaplegic homolog 4,MAD homolog 4,Mothers against DPP homolog 4,Deletion target in pancreatic carcinoma 4,SMAD family member 4,SMAD 4,Smad4,hSMAD4,SMAD4,DPC4, MADH4,

Background: SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFβ protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test ly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

Sequence Similarities: Belongs to the dwarfin/SMAD family.

Molekulargewicht: 60 kDa

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie