FADD Antikörper (C-Term)

Produktnummer ABIN3043202

Der Kaninchen Polyklonal Anti-FADD-Antikörper wurde für WB validiert. Er ist geeignet, FADD in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für FADD Antikörper (C-Term) (ABIN3043202)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FADD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-FADD Antikörper

Bindungsspezifität: AA 174-189, C-Term

Verwendungszweck: Anti-FADD Antibody Picoband®

Sequenz: ADLVQEVQQA RDLQNR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-FADD Antibody (ABIN3043202). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FADD.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kabra, N. H., Kang, C., Hsing, L. C., Zhang, J., Winoto, A.T cell-specific FADD-deficient mice: FADD is required for early T cell development.Proc. Nat. Acad. Sci. 98: 6307-6312, 2001. 2. Kim, P. K. M., Dutra, A. S., Chandrasekharappa, S. C., Puck, J. M.Genomic structure and mapping of human FADD, an intracellular mediator of lymphocyte apoptosis.J. Immun. 157: 5461-5466, 1996. 3. Lee, H.-J., Pyo, J.-O., Oh, Y., Kim, H.-J., Hong, S., Jeon, Y.-J., Kim, H., Cho, D.-H., Woo, H.-N., Song, S., Nam, J.-H., Kim, H. J., Kim, K.-S., Jung, Y.-K.AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10.Nature Cell Biol. 9: 1303-1310, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Andere Bezeichnung: FADD

Hintergrund:

Synonyms: FAS-associated death domain protein,FAS-associating death domain-containing protein,Growth-inhibiting gene 3 protein,Mediator of receptor induced toxicity,Protein FADD,FADD,MORT1,GIG3,

Tissue Specificity: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

Background: FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.

Sequence Similarities: Contains 1 death domain.

Molekulargewicht: 54 kDa

UniProt: Q13158

Pathways: Apoptose, TLR Signalweg, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades