Growth Hormone Receptor Antikörper (Middle Region)

Produktnummer ABIN3043066

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Growth Hormone Receptor in WB. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Growth Hormone Receptor Antikörper (Middle Region) (ABIN3043066)

Target: Growth Hormone Receptor (GHR)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Growth Hormone Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Growth Hormone Receptor Antikörper

Bindungsspezifität: AA 231-247, Middle Region

Verwendungszweck: Anti-Growth hormone receptor/GHR Antibody Picoband®

Sequenz: DKEHEVRVRS RQRSFEK

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Growth hormone receptor/GHR Antibody (ABIN3043066). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Growth hormone receptor, identical to the related rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Barton, D. E., Foellmer, B. E., Wood, W. I., Francke, U. : Chromosome mapping of the growth hormone receptor gene in man and mouse. Cytogenet. Cell Genet. 50: 137-141, 1989. 2. Godowski, P. J., Leung, D. W., Meacham, L. R., Galgani, J. P., Hellmiss, R., Keret, R., Rotwein, P. S., Parks, J. S., Laron, Z., Wood, W. I. : Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism. Proc. Nat. Acad. Sci. 86: 8083-8087, 1989. 3. Amselem, S., Sobrier, M.-L., Duquesnoy, P., Rappaport, R., Postel-Vinay, M.-C., Gourmelen, M., Dallapiccola, B., Goossens, M. : Recurrent nonsense mutations in the growth hormone receptor from patients with Laron dwarfism. J. Clin. Invest. 87: 1098-1102, 1991.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Growth Hormone Receptor

Target: Growth Hormone Receptor (GHR)

Andere Bezeichnung: GHR

Hintergrund:

Synonyms: Growth hormone receptor,GH receptor,Somatotropin receptor,Growth hormone-binding protein,GH-binding protein,GHBP,Serum-binding protein,Ghr,

Tissue Specificity: Expressed in all tissues tested including, liver, heart, adipose tissue, mammary gland, testes, ovary, brain, kidney and muscle. Highest levels in liver.

Background: The GHR locus to human chromosome 5p13.1-p12 and to mouse chromosome 15. Additionally, its gene has 9 exons that encode the receptor and several additional exons in the 5-prime untranslated region. The coding exons span at least 87 kb. GHR consists of an extracellular domain of 246 amino acids, a single transmembrane domain, and a cytoplasmic domain. Exons 3 to 7 encode the extracellular domain. There are 2 isoforms of GHR in humans, generated by retention or exclusion of exon 3 during splicing: a full-length isoform and an isoform that lacks exon 3 (d3GHR). Furthermore, the two isoforms of GHR are expressed in the placenta and appeared to be due to alternative splicing. In cirrhosis, there is a state of acquired GH resistance, as defined by high circulating GH levels with low IGF1 levels. Moreover, Mutations in the GHR gene have been demonstrated as the cause of Laron syndrome , also known as the growth hormone insensitivity syndrome (GHIS).

Sequence Similarities: Belongs to the type I cytokine receptor family. Type 1 subfamily.

Molekulargewicht: 72 kDa

UniProt: P16882

Pathways: NF-kappaB Signalweg, JAK-STAT Signalweg, Response to Growth Hormone Stimulus