JNK Antikörper (N-Term)

Produktnummer ABIN3043004

Der Kaninchen Polyklonal Anti-JNK-Antikörper wurde für WB validiert. Er ist geeignet, JNK in Proben von Human, Maus und Ratte zu detektieren. Es sind 7+ Publikationen verfügbar.

Kurzübersicht für JNK Antikörper (N-Term) (ABIN3043004)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JNK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-JNK Antikörper

Bindungsspezifität: AA 1-15, N-Term

Verwendungszweck: Anti-JNK1/MAPK8 Antibody Picoband®

Sequenz: MSRSKRDNNF YSVEI

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-JNK1/MAPK8 Antibody (ABIN3043004). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human JNK1, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Derijard, B., Hibi, M., Wu, I.-H., Barrett, T., Su, B., Deng, T., Karin, M., Davis, R. J.JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain.Cell 76: 1025-1037, 1994. 2. Gupta, S., Barrett, T., Whitmarsh, A. J., Cavanagh, J., Sluss, H. K., Derijard, B., Davis, R. J.Selective interaction of JNK protein kinase isoforms with transcription factors.EMBO J. 15: 2760-2770, 1996. 3. Tournier, C., Hess, P., Yang, D. D., Xu, J., Turner, T. K., Nimnual, A., Bar-Sagi, D., Jones, S. N., Flavell, R. A., Davis, R. J.Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway.Science 288: 870-874, 2000.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-JNK Antikörper (ABIN3043004)

Shangguan, Jiang, Pan, Xiao, Tan, Tie, Qin, Deng, Chen, Wang: "Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats." in: Cell death & disease, Vol. 8, Issue 10, pp. e3157, (2018) (PubMed).

Wang, Gao, Wu, Wang, Song, Chen, Zhang, Zhang, Zhang, Yu: "Pseudolaric acid B induced autophagy, but not apoptosis, in MRC5 human fibroblast cells." in: Oncology letters, Vol. 15, Issue 1, pp. 863-870, (2018) (PubMed).

Liu, Lv, Ning, Yang, Zhu: "Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model." in: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2284-2292, (2016) (PubMed).

Bu, Zhao, Zhang, Wang, Li, Yan: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." in: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).

Zheng, Liu, Liu, Ma, Zhou, Chen, Chang, Wang, Yang, He: "Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH?SY5Y human neuroblastoma cells." in: Molecular medicine reports, Vol. 10, Issue 1, pp. 89-94, (2014) (PubMed).

Ding, Zou, Li, Tian, Abdelalim, Du, She, Wang, Tan, Wang, Chen, Lv, Chang: "Study of histopathological and molecular changes of rat kidney under simulated weightlessness and resistance training protective effect." in: PLoS ONE, Vol. 6, Issue 5, pp. e20008, (2011) (PubMed).

Tian, Qiu, Zhao, Li, Guo: "Sphingomyelinase-induced ceramide production stimulate calcium-independent JNK and PP2A activation following cerebral ischemia." in: Brain injury, Vol. 23, Issue 13-14, pp. 1073-80, (2009) (PubMed).

Details zu JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Andere Bezeichnung: MAPK8

Hintergrund:

Synonyms: Mitogen-activated protein kinase 8,MAP kinase 8,MAPK 8,2.7.11.24,JNK-46,Stress-activated protein kinase 1c,SAPK1c,Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,MAPK8,JNK1, PRKM8, SAPK1, SAPK1C,

Background: MAPK8 (Mitogen-activated protein kinase 8), also known as JNK1, PRKM8, and SAPK1, is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase and JNK family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. This kinase is also involved in UV radiation-induced apoptosis, which is though to be related to the cytochrome c-mediated cell death pathway.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Molekulargewicht: 60 kDa

UniProt: P45983

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteine