NF-kB p65 Antikörper (N-Term)

Produktnummer ABIN3029745

Dieses Anti-NF-kB p65-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NF-kB p65 in WB. Geeignet für Human, Maus und Ratte.

Kurzübersicht für NF-kB p65 Antikörper (N-Term) (ABIN3029745)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human NF-kB p65 (AISQRIQTNNNPFQVP) was used as the immunogen for this p65 antibody (100% homologous in human and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the p65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: p65 NF-kB

Hintergrund: The p65 (RELA) heterodimer is the most abundant form of NF-KB. This gene is located on 11q13, which consists of 10 exons and spans about 8.1 kb of DNA. In rat sciatic nerves, the expression of the activated p65 subunit of NFKB was high in the nuclei of premyelinating Schwann cells and then progressively declined until it was nearly absent in adults. The transcriptional activity of NF-kappa-B is stimulated upon phosphorylation of its p65 subunit on serine-276 by protein kinase A(PKA). The transcriptional coactivator CBP/p300 associates with NF-kappa-B through 2 sites, an N-terminal domain that interacts with the C-terminal region of the unphosphorylated protein, and a second domain that only interacts with p65 phosphorylated on serine-276.

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine