Cyclin A Antikörper

Produktnummer ABIN3026638

Dieses Anti-Cyclin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A in IF und FACS. Geeignet für Human, Maus, Rind (Kuh), Green Monkey und Nerz.

Kurzübersicht für Cyclin A Antikörper (ABIN3026638)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Rind (Kuh), Green Monkey, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: E67

Produktdetails anti-Cyclin A Antikörper

Produktmerkmale: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Aufreinigung: Protein G affinity chromatography

Immunogen: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cyclin A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: Cyclin A2

Hintergrund: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Gen-ID: 890

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA