p21 Antikörper

Produktnummer ABIN3026466

Der Maus Monoklonal anti-p21 Antikörper wird verwendet zum Nachweis von p21 in Proben von Human, Maus und Ratte. Er wurde validiert für IF, FACS und IHC (p).

Kurzübersicht für p21 Antikörper (ABIN3026466)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: WA-1

Produktdetails anti-p21 Antikörper

Verwendungszweck: p21 Antibody (azide and preservative free)

Produktmerkmale: This antibody recognizes a 21 kDa protein, identified as the p21 or p21WAF1 tumor suppressor protein. This antibody is highly specific and shows no cross-reaction with other closely related mitotic inhibitors. p21 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. p21 expression is induced by the wild type, but not mutant, p53. Normal cells generally display a rather intense nuclear expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this p21 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p21 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the p21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: p21

Hintergrund: This antibody recognizes a 21 kDa protein, identified as the p21 or p21WAF1 tumor suppressor protein. This antibody is highly specific and shows no cross-reaction with other closely related mitotic inhibitors. p21 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. p21 expression is induced by the wild type, but not mutant, p53. Normal cells generally display a rather intense nuclear expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie