GTPase NRas Antikörper (Internal Region)
Kurzübersicht für GTPase NRas Antikörper (Internal Region) (ABIN302138)
Target
Alle GTPase NRas (NRAS) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- NRAS
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Sequenz
- RMKKLNSSDD GTQ
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Kreuzreaktivität
- Human, Maus, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
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Isotyp
- IgG
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Applikationshinweise
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Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
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Kommentare
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Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GTPase NRas (NRAS)
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Andere Bezeichnung
- NRAS
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Hintergrund
- NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog
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Gen-ID
- 4893, 18176, 24605
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NCBI Accession
- NP_002515
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Pathways
- p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
Target
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