TLR4 Antikörper (AA 100-200)

Produktnummer ABIN298531

Dieser Maus Monoklonal Antikörper erkennt spezifisch TLR4 in WB, FACS, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für TLR4 Antikörper (AA 100-200) (ABIN298531)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 76B357-1

Produktdetails anti-TLR4 Antikörper

Bindungsspezifität: AA 100-200

Marke: IHC-plus™

Spezifität: The amino acid sequence used has 100 % identity in human, chimp, baboon, Chinese hamster, and rat, 93 % identity in bovine and pig, 85 % identity in sheep and 78 % identity in cat, horse and mouse.

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide from human TLR4.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4

Hintergrund: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gen-ID: 7099

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine