PKC beta Antikörper (AA 607-671)
-
- Target Alle PKC beta (PRKCB) Antikörper anzeigen
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
-
Bindungsspezifität
- AA 607-671
-
Reaktivität
- Human, Ratte
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser PKC beta Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Spezifität
- Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
- Aufreinigung
- Affinity purified
- Immunogen
- E. coli-derived rhPKCb1 (aa607-671).
- Isotyp
- IgG
- Top Product
- Discover our top product PRKCB Primärantikörper
-
-
- Applikationshinweise
-
Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- deionized water
- Konzentration
- Lot specific
- Buffer
- Lyophilized.
-
- Target
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
- Andere Bezeichnung
- PRKCB / PKC-Beta (PRKCB Produkte)
- Synonyme
- PKC-beta antikoerper, PKCB antikoerper, PRKCB1 antikoerper, PRKCB2 antikoerper, A130082F03Rik antikoerper, PKC-Beta antikoerper, Pkcb antikoerper, Prkcb1 antikoerper, Prkcb2 antikoerper, PKC-B antikoerper, Prkcb antikoerper, prkcb1 antikoerper, zgc:63591 antikoerper, PKC antikoerper, PRKCB_tv2 antikoerper, Pkc53E antikoerper, prkcb1l antikoerper, zgc:64063 antikoerper, protein kinase C beta antikoerper, protein kinase C, beta antikoerper, protein kinase C, beta b antikoerper, protein kinase C, beta a antikoerper, PRKCB antikoerper, Prkcb antikoerper, prkcbb antikoerper, prkcba antikoerper
- Hintergrund
-
Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II - Gen-ID
- 5579
- UniProt
- P05771
- Pathways
- WNT Signalweg, T-Zell Rezeptor Signalweg, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
-