PKC beta Antikörper (AA 607-671)

Produktnummer ABIN296089

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PKC beta in WB, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für PKC beta Antikörper (AA 607-671) (ABIN296089)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PKC beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-PKC beta Antikörper

Bindungsspezifität: AA 607-671

Spezifität: Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rhPKCb1 (aa607-671).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Andere Bezeichnung: PRKCB / PKC-Beta

Hintergrund: Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II

Gen-ID: 5579

UniProt: P05771

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling