TNF alpha Antikörper

Produktnummer ABIN289560

Dieses Anti-TNF alpha-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TNF alpha in WB, ELISA und Inhibition. Geeignet für Maus. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für TNF alpha Antikörper (ABIN289560)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Produktdetails anti-TNF alpha Antikörper

Aufreinigung: Purified

Immunogen: TNF alpha antibody was raised in rabbit using highly pure recombinant murine TNF-alpha as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 0.5-2 µg/mL, Inhibition: 0.04-0.05 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in water to a concentration 0.1 - 1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Referenzen für anti-TNF alpha Antikörper (ABIN289560)

McCullough, McMullen, Poulsen, Kim, Medof, Nagy: "Anaphylatoxin Receptors C3aR and C5aR1 Are Important Factors That Influence the Impact of Ethanol on the Adipose Secretome." in: Frontiers in immunology, Vol. 9, pp. 2133, (2019) (PubMed).

McCullough, McMullen, Das, Roychowdhury, Strainic, Medof, Nagy: "Differential contribution of complement receptor C5aR in myeloid and non-myeloid cells in chronic ethanol-induced liver injury in mice." in: Molecular immunology, Vol. 75, pp. 122-32, (2017) (PubMed).

Zhuge, Cederbaum: "Inhibition of the mitochondrial permeability transition by cyclosporin A prevents pyrazole plus lipopolysaccharide-induced liver injury in mice." in: Free radical biology & medicine, Vol. 46, Issue 3, pp. 406-13, (2009) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF alpha

Hintergrund: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Molekulargewicht: 17.3 kDa (predicted detection band MW)

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome