TNF alpha Antikörper

Produktnummer ABIN289547

Der Ziege Polyklonal Anti-TNF alpha-Antikörper wurde für WB, ELISA und Inhibition validiert. Er ist geeignet, TNF alpha in Proben von Human zu detektieren.

Kurzübersicht für TNF alpha Antikörper (ABIN289547)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Produktdetails anti-TNF alpha Antikörper

Aufreinigung: Purified

Immunogen: TNF alpha antibody was raised in goat using highly pure recombinant human TNF-alpha as the immunogen.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA: 0.5-2 µg/mL, Inhibition: 0.012-0.014 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of > 0.2 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from 0.5 x PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF alpha

Hintergrund: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Molekulargewicht: 17.3 kDa (predicted detection band MW)

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome