Cyclin A Antikörper

Produktnummer ABIN2855543

Der Kaninchen Polyklonal Anti-Cyclin A-Antikörper wurde für WB, IF, IP, IHC (p) und ICC validiert. Er ist geeignet, Cyclin A in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für Cyclin A Antikörper (ABIN2855543)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-Cyclin A Antikörper

Spezifität: IP/MS validation was supported by references (PMID:30377384)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Cyclin A2 (cyclin A2)
Cyclin A2 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro2A , PC-12 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr)

Validation: IP/MS, KO/KD, Orthogonal, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.73 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-Cyclin A Antikörper (ABIN2855543)

Honda, Inui: "PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression." in: Scientific reports, Vol. 10, Issue 1, pp. 1140, (2020) (PubMed).

Ogawa, Yaginuma, Nakatomi, Nakajima, Tada-Shigeyama, Addison, Urata, Matsubara, Watanabe, Matsuo, Sato, Honda, Hikiji, Watanabe, Kokabu: "Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity." in: Oncotarget, Vol. 10, Issue 3, pp. 404-414, (2019) (PubMed).

Fu, Xu, Chen, Li, Zhao, Chen: "Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 4337-4344, (2018) (PubMed).

Li, Wu, Lee, Li, Hsieh, Ho, Ho, Chen, Wang, Yen, Yang, Chen: "Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e104203, (2015) (PubMed).

Lin, Chou, Lee, Chen, Fong, Chang, Huang, Wu, Chang, Wang, Chiu: "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB." in: Cancer cell international, Vol. 14, Issue 1, pp. 1, (2014) (PubMed).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: cyclin A2

Hintergrund: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Cellular Localization: Nucleus , Cytoplasm

Molekulargewicht: 49 kDa

Gen-ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA