NF-kB p65 Antikörper (Center)

Produktnummer ABIN2855287

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NF-kB p65 in WB, IF, IHC (p), ICC und ChIP. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für NF-kB p65 Antikörper (Center) (ABIN2855287)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Rind (Kuh), Schwein, Ratte (Rattus)

Kreuzreaktivität (Details): Pig (98 %), Rat (99 %), Bovine (98 %)

Produktmerkmale: Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
NFkB p65 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Kommentare:

Positive Control: A431 , HepG2 , human NFkB p65-transfected 293T

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.03 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NF-kB p65 Antikörper (ABIN2855287)

Arellano Buendía, Tostado González, Sánchez Reyes, García Arroyo, Argüello García, Tapia, Sánchez Lozada, Osorio Alonso: "Immunomodulatory Effects of the Nutraceutical Garlic Derivative Allicin in the Progression of Diabetic Nephropathy." in: International journal of molecular sciences, Vol. 19, Issue 10, (2019) (PubMed).

Chen, Kuo, Liao, Lin, Tien, Huang: "Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway." in: Oncogene, Vol. 37, Issue 46, pp. 6041-6053, (2019) (PubMed).

Menicacci, Cipriani, Margheri, Mocali, Giovannelli: "Modulation of the Senescence-Associated Inflammatory Phenotype in Human Fibroblasts by Olive Phenols." in: International journal of molecular sciences, Vol. 18, Issue 11, (2018) (PubMed).

Chamard-Jovenin, Thiebaut, Chesnel, Bresso, Morel, Smail-Tabbone, Devignes, Boukhobza, Dumond: "Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells." in: Frontiers in endocrinology, Vol. 8, pp. 272, (2017) (PubMed).

Thiebaut, Chamard-Jovenin, Chesnel, Morel, Djermoune, Boukhobza, Dumond: "Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression." in: PLoS ONE, Vol. 12, Issue 3, pp. e0173931, (2017) (PubMed).

Wu, Hwang-Verslues, Lee, Huang, Wei, Chen, Shew, Lee, Jeng, Tien, Ma, Lee: "Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines." in: The Journal of experimental medicine, Vol. 212, Issue 3, pp. 333-49, (2015) (PubMed).

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: NFkB p65

Hintergrund: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Nucleus , Cytoplasm

Molekulargewicht: 60 kDa

Gen-ID: 5970

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine