PRKAG1 Antikörper (Center)

Produktnummer ABIN2855115

Dieses Anti-PRKAG1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PRKAG1 in WB und IP. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PRKAG1 Antikörper (Center) (ABIN2855115)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-PRKAG1 Antikörper

Bindungsspezifität: Center

Kreuzreaktivität: Schwein, Rhesusaffen, Rind (Kuh)

Kreuzreaktivität (Details): Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to AMPK gamma 1 (5'-AMP-activated protein kinase subunit gamma-1)
AMPK gamma 1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human AMPK gamma 1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference Immunoprecipitation Assay-dependent dilution Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitationAssay-dependent dilution Western blot1:500-1:3000*

Kommentare:

Positive Control: mouse liver

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.33 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PRKAG1 Antikörper (ABIN2855115)

Lin, Kiihl, Suhail, Liu, Chou, Kuang, Lu, Khor, Lin, Bader, Irizarry, Boeke: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).

Details zu PRKAG1

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Andere Bezeichnung: AMPK gamma 1

Hintergrund: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molekulargewicht: 38 kDa

Gen-ID: 5571

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effekt