PCNA Antikörper

Produktnummer ABIN2854788

Dieser Anti-PCNA Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von PCNA in WB, IHC, IF, IP, IHC (p), ICC, IHC (fro) und FISH. Geeignet für Human. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für PCNA Antikörper (ABIN2854788)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence in situ hybridization (FISH)

Produktdetails anti-PCNA Antikörper

Spezifität: IP/MS validation was supported by references (PMID:30377373)

Kreuzreaktivität: Syrischer Goldhamster, Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)
PCNA antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: NIH-3T3 , PC-12

Validation: IP/MS, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.11 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PCNA Antikörper (ABIN2854788)

Mousa, Alhumaydhi, Abdellatif, Abdulmonem, AlKhowailed, Alsagaby, Al Rugaie, Alnuqaydan, Aljohani, Aljasir, Alwashmi, Soliman, Yosof, Elsheikh, Babiker, Alsuhaibani, Hegazy, Seleem: "Curcumin and ustekinumab cotherapy alleviates induced psoriasis in rats through their antioxidant, anti-inflammatory, and antiproliferative effects." in: Cutaneous and ocular toxicology, pp. 1-10, (2021) (PubMed).

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Chen, Chen, Lin, Hsieh, Hsu, Hsieh: "Synergistic Anticancer Effects of Gemcitabine with Pitavastatin on Pancreatic Cancer Cell Line MIA PaCa-2 in vitro and in vivo." in: Cancer management and research, Vol. 12, pp. 4645-4665, (2020) (PubMed).

Hsieh, Wang, Changou, Chin, Yang, Lai, Lee, Yang, Whang-Peng, Liu, Lin, Mousa, Davis: "Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells." in: Oncotarget, Vol. 8, Issue 15, pp. 24237-24249, (2018) (PubMed).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: proliferating cell nuclear antigen

Hintergrund: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Cellular Localization: Nucleus

Molekulargewicht: 29 kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie