Cyclin A Antikörper (Biotin)

Produktnummer ABIN2853583

Dieses Anti-Cyclin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A in WB und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Cyclin A Antikörper (Biotin) (ABIN2853583)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: E67-1

Produktdetails anti-Cyclin A Antikörper (Biotin)

Spezifität: This antibody reacts with Cyclin A (55 kDa).

Aufreinigung: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Bovine recombinant Cyclin A.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 20 µg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: Cyclin A2

Hintergrund: Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21CIPP1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcrIPPtion factors DRT F1 and E2F, and retinoblastoma protein, p110.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2

Gen-ID: 890

NCBI Accession: NP_001228

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA