HCLS1 Antikörper (N-Term)

Produktnummer ABIN2779751

Dieser Anti-HCLS1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von HCLS1 in WB. Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Meerschweinchen, Schwein und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für HCLS1 Antikörper (N-Term) (ABIN2779751)

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Kaninchen, Meerschweinchen, Schwein, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCLS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HCLS1 Antikörper

Bindungsspezifität: N-Term

Sequenz: FGVERDRMDK SAVGHEYVAE VEKHSSQTDA AKGFGGKYGV ERDRADKSAV

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against HCLS1. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HCLS1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 486 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-HCLS1 Antikörper (ABIN2779751)

Ruzzene, Penzo, Pinna: "Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells." in: The Biochemical journal, Vol. 364, Issue Pt 1, pp. 41-7, (2002) (PubMed).

Details zu HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Andere Bezeichnung: HCLS1

Hintergrund: HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.
Alias Symbols: HS1, CTTNL
Protein Interaction Partner: SH2D4A, UBC, Mbp, Dlg4, NOTCH1, QKI, TERF1, TRIM29, SSBP3, BLZF1, IKBKG, LZTR1, ZBTB25, HS1BP3, HAX1, ACTR2, MAP4K1, CASP3, SYK, WAS, LYN, FGR, CSNK2A1, CD79A, ACTA1, GRB2, ACTR3,
Protein Size: 486

Molekulargewicht: 54 kDa

Gen-ID: 3059

NCBI Accession: NM_005335, NP_005326

UniProt: P14317

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location