Caspase 8 Antikörper

Produktnummer ABIN2745526

Dieses Anti-Caspase 8-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Caspase 8 in WB, IHC, ELISA, ICC und FACS. Geeignet für Maus.

Kurzübersicht für Caspase 8 Antikörper (ABIN2745526)

Target: Caspase 8 (CASP8)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 3B10

Produktdetails anti-Caspase 8 Antikörper

Spezifität: Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55 kDa (full-length caspase-8) and ~18 kDa (apoptosis-induced cleavage fragment) by Western blot.

Kreuzreaktivität: Maus

Keine Kreuzreaktivität: Human

Kreuzreaktivität (Details): Does not cross-react with human caspase-8.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse caspase-8 (p20 subunit).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: Caspase-8

Hintergrund: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

UniProt: O89110

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades