Cyclin A Antikörper

Produktnummer ABIN2740254

Der Maus Monoklonal Anti-Cyclin A-Antikörper wurde für WB, IHC, IF, ELISA, FACS, IP und IHC (p) validiert. Er ist geeignet, Cyclin A in Proben von Human, Maus, Rind (Kuh), Green Monkey und Nerz zu detektieren.

Kurzübersicht für Cyclin A Antikörper (ABIN2740254)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Rind (Kuh), Green Monkey, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: E67

Produktdetails anti-Cyclin A Antikörper

Spezifität: Bovine Cyclin A1+2

Aufreinigung: Protein G purified

Immunogen: Full length bovine protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (1 - 2 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a protein of 54  kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase. ELISA: Order BSA-free antibody format for coating.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2°C to 8°C.

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: Cyclin A

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA