MDM2 Antikörper

Produktnummer ABIN271933

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MDM2 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für MDM2 Antikörper (ABIN271933)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MDM2 Antikörper

Spezifität: This antibody detects endogenous levels of MDM2 protein. (region surrounding Ser160)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: MDM2

Hintergrund: P53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Molekulargewicht: approx. 55, 90 kDa

Gen-ID: 4193

NCBI Accession: NP_002383

UniProt: Q00987

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway