Dieser TNF alpha Antikörper ist konjugiert mit Biotin
Applikation
ELISA
Marke
BD Pharmingen™
Produktmerkmale
The 516D1A1 Fab antibody reacts with mouse and rat tumor-necrosis factor (TNF, formerly known as TNF-α) proteins. The 516D1A1 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with recombinant mouse TNF protein. The biology and function of the TNF protein has been extensively reviewed (Hehlgans et al.) in the literature. The biotinylated 516D1A1 antibody is useful as a detection antibody for a sandwich ELISA that measures mouse and rat TNF protein levels. Purified Hamster Anti-Mouse/Rat TNF (Cat. No.557516) as capture antibody can be paired with biotinylated 516D1A1 as the detection antibody, using purified recombinant mouse TNF protein (Cat. No. 554589) or purified recombinant rat TNF protein (Cat. No. 555109) as standard. The biotinylated 516D1A1 antibody should be titrated between 0.25 - 2.0 μg/mL to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse or rat TNF protein ranging from 31.25 pg/mL to 2,000 pg/mL are recommended for inclusion in each ELISA plate. Investigators may find methodology information to be useful in Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences (2003)-- Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. For this ELISA antibody pair, no cross-reactivity has been observed with the following: Rat IL-1α, IL-2, IL-4, IL-6, IL-10, IL-18, GM-CSF, IFN-γ, mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-18, M-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, sTNFRI, sTNFRII, RANTES, human TNF. Mouse TNF sandwich ELISA curve: The curve was generated by sandwich ELISA using the purified TN3-19.12 (Cat. No. 557516) as capture antibody, doubling dilutions of recombinant mouse TNF protein (Cat. No. 554589) and biotin 516D1A1 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.
BD Pharmingen™ Biotin Human anti-Mouse/Rat TNF - Biotin - Clone 516D1A1 - Isotype Human Ig, κ - Reactivity Ms, Rat - 0.5 mg
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Hehlgans, Pfeffer: "The intriguing biology of the tumour necrosis factor/tumour necrosis factor receptor superfamily: players, rules and the games." in: Immunology, Vol. 115, Issue 1, pp. 1-20, (2005) (PubMed).