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CD11b Antikörper

ITGAM Reaktivität: Human, Maus FACS, WB, IP, IHC (fro), IHC (p), BR Wirt: Ratte Monoclonal M1-70 unconjugated
Produktnummer ABIN2688906
  • Target Alle CD11b (ITGAM) Antikörper anzeigen
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Reaktivität
    • 279
    • 178
    • 70
    • 33
    • 17
    • 17
    • 16
    • 14
    • 11
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 1
    Human, Maus
    Wirt
    • 155
    • 113
    • 105
    • 4
    • 1
    • 1
    Ratte
    Klonalität
    • 279
    • 96
    • 1
    Monoklonal
    Konjugat
    • 125
    • 37
    • 32
    • 28
    • 21
    • 11
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD11b Antikörper ist unkonjugiert
    Applikation
    • 279
    • 87
    • 79
    • 78
    • 63
    • 43
    • 38
    • 27
    • 23
    • 20
    • 13
    • 13
    • 11
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)
    Marke
    BD Pharmingen™
    Produktmerkmale
    The M1/70 antibody reacts with the 170 kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β2 integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11b on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified Rat IgG2b, κ isotype control A95-1 (Cat. No. 553986, left panel) or purified M1/70 monoclonal antibodies (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

    BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms, Hu - 0.5 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes
    Klon
    M1-70
    Isotyp
    IgG2b kappa
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

    Lub, van Kooyk, Figdor: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." in: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, (1996) (PubMed).

    Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, (1989) (PubMed).

    Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, (1983) (PubMed).

    Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, (1983) (PubMed).

    Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).

    Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, (1979) (PubMed).

    Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, (1978) (PubMed).

  • Target
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Andere Bezeichnung
    CD11b (ITGAM Produkte)
    Synonyme
    CD11b/CD18 antikoerper, CR3 antikoerper, CR3A antikoerper, Cd11b antikoerper, F730045J24Rik antikoerper, Ly-40 antikoerper, MAC1 antikoerper, Mac-1 antikoerper, Mac-1a antikoerper, CD11B antikoerper, MAC-1 antikoerper, MAC1A antikoerper, MO1A antikoerper, SLEB6 antikoerper, C3bi antikoerper, integrin subunit alpha M antikoerper, integrin alpha M antikoerper, integrin, alpha M (complement component 3 receptor 3 subunit) antikoerper, integrin alpha-M antikoerper, ITGAM antikoerper, Itgam antikoerper, LOC100351865 antikoerper
    Hintergrund
    Synonyms: Integrin α[M] chain, Mac-1 α chain, CR3
    Pathways
    Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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