NF-kB p65 Antikörper (AA 526-539)

Produktnummer ABIN2668795

Es gibt 1 Publikation für dieses Produkt. Der Maus Monoklonal anti-NF-kB p65 Antikörper wird verwendet zum Nachweis von NF-kB p65 in Proben von Human und Maus. Er wurde validiert für WB und ChIP.

Kurzübersicht für NF-kB p65 Antikörper (AA 526-539) (ABIN2668795)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Klon: 112A1021

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: AA 526-539

Verwendungszweck: NFkB p65 antibody (mAb) (Clone 112A1021)

Aufreinigung: Affinity Purified

Immunogen: This NFkB p65 antibody was raised against a synthetic peptide corresponding to amino acid residues 526-539 of human NFkB p65.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 2 - 5 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-NF-kB p65 Antikörper (ABIN2668795)

Sun, Alkhoury, Wang, Foster, Radecke, Tam, Edwards, Facciotti, Armstrong, Knowlton, Newman, Passerini, Simon: "IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal." in: Circulation research, Vol. 111, Issue 8, pp. 1054-64, (2012) (PubMed).

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: NFkappaB p65

Hintergrund: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Molekulargewicht: 65 kDa

Gen-ID: 5970

NCBI Accession: NP_068810

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine