TNF alpha Antikörper (Biotin)

Produktnummer ABIN264362

Der Maus Monoklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Human und Affe. Er wurde validiert für EIA.

Kurzübersicht für TNF alpha Antikörper (Biotin) (ABIN264362)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist konjugiert mit Biotin

Applikation: Enzyme Immunoassay (EIA)

Klon: AS1

Produktdetails anti-TNF alpha Antikörper (Biotin)

Produktmerkmale: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Aufreinigung: Protein G Chromatography

Reinheit: > 95 % by SDS-PAGE

Immunogen: Genename: TNF

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: This antibody has been used as a solution phase antibody at 2 μg/mL in a two site ELISAwith unconjugated TNFalpha antibody (Cat. No. ABIN264365). Typically the ELISA has aworking range of 0-10000 pg/mL and a sensitivity of 10 pg/mL with the appropriatestandard. Recommended sample volumes are 25-200 μL. Recovery of human TNFa in human serum is typically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor (TNF-Alpha)

Hintergrund: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome