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CD11b Antikörper (FITC)

ITGAM Reaktivität: Human FACS Wirt: Maus Monoclonal F6-2 FITC

Produktnummer ABIN264313

Datenblatt (PDF)

Target Alle CD11b (ITGAM) Antikörper anzeigen

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Reaktivität
274

177

74

34

20

19

17

14

11

10

7

4

4

2

2

1
Human
Wirt
157

116

105

2

1

1
Maus
Passende Sekundärantikörper
Klonalität
284

94

2
Monoklonal
Konjugat
125

39

33

29

21

10

8

8

7

7

6

6

5

5

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Dieser CD11b Antikörper ist konjugiert mit FITC
Applikation
285

87

86

82

64

49

38

24

23

21

14

14

12

6

5

5

5

4

4

3

2

2

2

2

1

1

1

1
Flow Cytometry (FACS)

Produktmerkmale

Synonyms: Integrin alpha-M, CR3A, MAC-1, MAC1, CR-3 alpha chain, Cell surface glycoprotein MAC-1subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11antigen-like family member B

Klon

F6-2

Isotyp

IgG2a

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anti-Integrin alpha M (ITGAM) antibody (FITC)
ITGAM Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-174 FITC

anti-Integrin alpha M (ITGAM) antibody (Biotin)
ITGAM Reaktivität: Human FACS, IP Wirt: Maus Monoclonal MEM-174 Biotin

anti-Integrin alpha M (ITGAM) antibody (PE)
ITGAM Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-174 PE

anti-Integrin alpha M (ITGAM) (AA 941-1074) antibody
ITGAM Reaktivität: Human IHC, WB, StM Wirt: Maus Monoclonal ITGAM-3338 unconjugated

anti-Integrin alpha M (ITGAM) (AA 623-728) antibody
ITGAM Reaktivität: Human ELISA, ICC Wirt: Maus Monoclonal 3A10H5 unconjugated

Applikationshinweise

Flow Cytometry. Monitoring of subsets in peripheral blood. Characterization of leukemias and lymphomas. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll

PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Buffer

Sterile filtered PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Haltbarkeit

12 months
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung

CD11b (ITGAM Produkte)

Hintergrund

(Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30 % of peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gen-ID

3684

UniProt

P11215

Pathways

Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation