PRKACA Antikörper

Produktnummer ABIN238374

Produktdetails anti-PRKACA Antikörper

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRKACA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Produktmerkmale: Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Aufreinigung: Protein A affinity chromatography.

Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).

Klon: 6D2-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Western Blotting (0.5-1 μg/mL). Positive control: HeLa human cervix carcinoma cell lineNote: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing thissubunit (testis).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu PRKACA

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Andere Bezeichnung: PRKACA (PRKACA Produkte)

Synonyme: PKACA antikoerper, Calpha antikoerper, Cs-PKA antikoerper, PKCA1 antikoerper, PKA antikoerper, PKCD antikoerper, Pkaca antikoerper, zgc:158799 antikoerper, protein kinase cAMP-activated catalytic subunit alpha antikoerper, protein kinase, cAMP dependent, catalytic, alpha antikoerper, cAMP-dependent protein kinase catalytic subunit antikoerper, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a antikoerper, PRKACA antikoerper, Prkaca antikoerper, kin-1 antikoerper, prkacaa antikoerper

Hintergrund: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Gen-ID: 5566

UniProt: P17612

Pathways: NF-kappaB Signalweg, Hedgehog Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion