c-MET Antikörper (pTyr1234, Tyr1235)
Kurzübersicht für c-MET Antikörper (pTyr1234, Tyr1235) (ABIN224750)
Target
Alle c-MET (MET) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Bindungsspezifität
- pTyr1234, Tyr1235
-
Spezifität
- Detects endogenous human HGF R/c-MET phosphorylated at Y1234/Y1235.
-
Aufreinigung
- Immunoaffinity purified
-
Immunogen
- Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
-
Approved: Flo, WB
Usage: Western Blot: An antibody concentration of 0.5 μg/mL is recommended. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 L. Following a 30 minute incubation, cells should be washed in an isotonic phosphate buffer (supplemented with 0.5 % BSA) prior to the addition of a secondary developing reagent. The binding of unlabeled polyclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as anti-rabbit IgG conjugated to a fluorochrome. Cells should be washed for a final time prior to flow cytometric analysis. -
Kommentare
-
Target Species of Antibody: Human
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Lyophilized
-
Rekonstitution
- deionized water
-
Konzentration
- Lot specific
-
Buffer
- Lyophilized.
-
-
- c-MET (MET) (Met Proto-Oncogene (MET))
-
Andere Bezeichnung
- c-Met
-
Hintergrund
-
Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met -
Gen-ID
- 4233
-
UniProt
- P08581
-
Pathways
- RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
Target
-