ERK1 Antikörper (pThr202, Tyr204)

Produktnummer ABIN220153

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ERK1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für ERK1 Antikörper (pThr202, Tyr204) (ABIN220153)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ERK1 Antikörper

Bindungsspezifität: pThr202, Tyr204

Spezifität: Specific for ERK 1+2 when phosphorylated at Thr202 and Tyr204. On Western blots, it identifies two bands at ~42 and 44kD. Reactivity has been confirmed with UV-treated human Jurkat cell lysates. Immunolabeling in UV-treated Jurkat cells is blocked by the phosphopeptide, but not by the dephosphopeptide.

Aufreinigung: Purified

Immunogen: Synthetic phosphopeptide corresponding to the regions surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).

Type of Immunogen: Synthetic phospho-peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot (rat): 1:1000. Immunohistochemistry, human (formalin fixed, paraffin embedded): 1:400. Staining of formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval with citrate buffer,  pH 6.0, is required prior to staining.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES solution,  pH 7.5, 0.1 mg/mL BSA, 50 % glycerol.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3 / ERK1

Hintergrund: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gen-ID: 5595

UniProt: P27361

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine