CD36 Antikörper

Produktnummer ABIN2191823

Der Maus Monoklonal Anti-CD36-Antikörper wurde für FACS, WB, IF, IP und Func validiert. Er ist geeignet, CD36 in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD36 Antikörper (ABIN2191823)

Target: CD36

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD36 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

Klon: CRF D-2712

Produktdetails anti-CD36 Antikörper

Endotoxin-Niveau: Low endotoxin level

Anwendungsinformationen

Applikationshinweise: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:25. For functional studies dilutions have to be made according to the amounts of CD36 binding to be blocked.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 2 mL(> 100 μg/mL) culture medium with low endotoxin level.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-CD36 Antikörper (ABIN2191823)

Bastie, Nahlé, McLoughlin, Esser, Zhang, Unterman, Abumrad: "FoxO1 stimulates fatty acid uptake and oxidation in muscle cells through CD36-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14222-9, (2005) (PubMed).

Kunjathoor, Febbraio, Podrez, Moore, Andersson, Koehn, Rhee, Silverstein, Hoff, Freeman: "Scavenger receptors class A-I/II and CD36 are the principal receptors responsible for the uptake of modified low density lipoprotein leading to lipid loading in macrophages." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49982-8, (2002) (PubMed).

Smith, Serghides, Patel, Febbraio, Silverstein, Kain: "CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum." in: Infection and immunity, Vol. 71, Issue 1, pp. 393-400, (2002) (PubMed).

Finnemann, Silverstein: "Differential roles of CD36 and alphavbeta5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1289-98, (2001) (PubMed).

Details zu CD36

Target: CD36

Andere Bezeichnung: CD36

Hintergrund: Monoclonal antibody CRF D-2712 (also known as JC63.1 or 63) reacts with mouse CD36 (85- kDa), a cell surface class B scavenger receptor. Recently, CD36 has been identified as a protein that is required for TLR2 recognition of di-acylated bacterial lipopeptides and lipoteichoic acid. CD36 is a heavily N- glycosylated transmembrane protein with two short intracellular domains and a large extracellular domain. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adaptor molecules. CD36 is expressed on monocytes, macrophages, endothelial cells, hepatocytes, erythrocytes, adipocytes, cardiomyocytes and red skeletal muscle. Importantly it is expressed by steriodogenic cells in the adrenal gland, testis and ovary. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. CD36 is associated with Src-family kinases and with the integrins alpha3beta1 and alpha6beta1. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been shown to function as phagocytic receptor for apoptotic cells. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum- infected red blood cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues. Monoclonal antibody CRF D-2712 blocks CD36 binding. It is not known whether the antibody is activating or neutralizing. The antibody cross reacts with rat CD36.

Pathways: TLR Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteine