TLR9 Antikörper

Produktnummer ABIN2191784

Der Maus Monoklonal Anti-TLR9-Antikörper wurde für WB, FACS, IHC (p), IHC (fro), IA und D validiert. Er ist geeignet, TLR9 in Proben von Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für TLR9 Antikörper (ABIN2191784)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA), Detection (D)

Klon: 5G5

Produktdetails anti-TLR9 Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-TLR9 Antikörper (ABIN2191784)

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, (2008) (PubMed).

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, (2004) (PubMed).

Details zu TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Andere Bezeichnung: Toll-Like Receptor 9

Hintergrund: The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG- dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades