TNF alpha Antikörper

Produktnummer ABIN2191760

Der Ratte Monoklonal Anti-TNF alpha-Antikörper wurde für FACS und Func validiert. Er ist geeignet, TNF alpha in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für TNF alpha Antikörper (ABIN2191760)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func)

Klon: V1q

Produktdetails anti-TNF alpha Antikörper

Kreuzreaktivität (Details): Cross reactivity: Chinese hamster TNF-α : Yes, Receptor-bound mouse TNF-α : No

Sterilität: 0.2 μm filtered

Isotyp: IgD

Anwendungsinformationen

Applikationshinweise: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Monocytes/macrophages control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Referenzen für anti-TNF alpha Antikörper (ABIN2191760)

Rajashekhar, Grow, Willuweit, Patterson, Clauss: "Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation." in: Physiological genomics, Vol. 31, Issue 1, pp. 104-13, (2007) (PubMed).

Demjen, Klussmann, Kleber, Zuliani, Stieltjes, Metzger, Hirt, Walczak, Falk, Essig, Edler, Krammer, Martin-Villalba: "Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury." in: Nature medicine, Vol. 10, Issue 4, pp. 389-95, (2004) (PubMed).

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).

Echtenacher, Falk, Männel, Krammer: "Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3762-6, (1991) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor alpha

Hintergrund: The monoclonal antibody V1q recognizes mouse tumor necrosis factor alpha (TNF-α). TNF-α is the prototype cytokine of the family of TNF-related ligands, which are based on structural and functional homologies. TNF-α is synthesized as type II transmembrane protein. TNF-α can be recognized by two different membrane receptors, namely TNF-R1 and TNF-R2. TNF-α is present in a membrane-bound (tmTNF) as well as soluble form (sTNF). The membrane-bound form of TNF-α is recognized by both TNF receptors with high affinity, whereas the soluble form is recognized more superiorly by TNF-R1. TNF-α is produced by many different cell types including macrophages, T lymphocytes, NK cells, neutrophils and endothelial cells. Cells differ in the expression of the two TNF-receptors and sTNF versus tmTNF, respectively. TNF-α, a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-α has been implied as central mediator in shock induced by gram negative micro-organisms. TNF-α induces on its turn the production of many other cytokines. Furthermore, TNF-α has been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The monoclonal antibody V1q recognizes both natural and recombinant TNF-α and shows neutralizing activity. Aliases TNF, TNF-SF2, DIF, cachectin, TNF-α, tumor necrosis factor ligand superfamily member 2 Immunogen Cytotoxin purified from conA induced T cell clone 29

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome